SpectraMax 190

type: 
Spectrometers / Spectrophotometers UV and visible region
manufacturer: 
SpectraMax
Country of origin: 
England
appointment: 
The patented multichannel optical design of the SpectraMax 190 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measures each sample in the plate directly, and eliminates measurement error due to variations in light output between optical fibers on other instruments for more reliable data reporting.
technical characteristics: 

Wavelength Range                                        190-850 nm

Wavelength Selection                                    Monochromator, tunable 1/0 nm increments

Wavelength Bandwidth                                 ≤ 2.0 nm

Wavelength Accuracy                                    ± 1.0 nm

Wavelength Repeatability                             ± 0.2 nm

Photometric Range                                       0.0 to 4.0 OD

Photometric  Resolution                                0.001 OD

Photometric Accuracy                                   < ± 0.006 OD ± 1.0%, 0.0 - 2.0 OD

Photometric Precision                                   < ± 0.003 OD ± 1.0%, 0.0 - 2.0 OD

Stray Light                                                     < 0.05% @ 230 nm

Light Source                                                   Xenon flash lamp

Microplate Read Time — Endpoint  9 seconds

Microplate Read Time — Kinetic                 9 second minimum interval

Temperature Range                                      Ambient + 4°C up to 45°C

Temperature Uniformity                               ± 0.5°C @ 37°C

description: 

Benefits

  • Eliminate the need for purchasing and changing out filters for wavelength optimization with a monochromator-based wavelength selection for UV-visible absorbance between 190 nm and 850 nm
  • Read up to 6 wavelengths in endpoint or kinetic mode for 96-well plates, and perform spectral scans to determine wavelength peaks
  • Measurement accuracy is increased due to narrow bandwidths that deliver increased and linearity over the widest range of assays, including DNA quantitation in the UV range
  • Better experimental signal discrimination using narrow absorbance bandpass for superior peak resolution and increased accuracy
  • Quickly quantify DNA, RNA, and protein samples as low as 2 µL with the SpectraDrop Micro-Volume Microplate

Features

  • Ability to monitor temperature-sensitive reactions with consistent temperature regulation from ambient to 45°C
  • Correction for varying microplate well volumes and the elimination of standard curves based on PathCheck® Technology, the only temperature-independent pathlength correction method
  • Thorough mixing of samples in microplate wells by user-programmable Automix feature
  • Seamless automation coupling with the StakMax Stacker, Molecular Devices' own integrated microplate stacker designed exclusively for the SpectraMax Reader